| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Tropheryma whipplei DNA Qualitative,Real-Time PCR
Test Code11352
Alias/See Also
LAB15337
Preferred Specimen
> 3 mm3 biopsy, submitted in a sterile, plastic leak-proof container, or
0.7 mL CSF or synovial fluid collected in a sterile, leak-proof container, or
0.7 mL whole blood collected in an EDTA (lavender-top) tube
0.7 mL CSF or synovial fluid collected in a sterile, leak-proof container, or
0.7 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
3 mm3 frozen tissue • 0.3 mL CSF, synovial fluid or whole blood
Transport Temperature
Frozen
Specimen Stability
Biopsy and synovial fluid
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days
Whole blood and CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days
Whole blood and CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Set up: Mon-Sat; Report available: 2-3 days
Clinical Significance
Tropheryma whipplei (Whipple's disease) DNA, PCR is a highly sensitive and specific method to detect the presence of Tropheryma whipplei DNA in clinical specimens. The diagnosis of Tropheryma whipplei infection should not rely solely upon the result of a PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Tropheryma whipplei DNA at detectable levels in the samples tested and does not exclude the diagnosis of disease.
