MGMT Promoter Methylation Tumor

Test Code

37374

Alias/See Also

LAB1234366

Includes

Note: When this test is ordered, slide review will always be performed at an additional charge (CPT 88381). Pathology report must accompany specimen in order for testing to be performed.

Preferred Specimen

Formalin-fixed paraffin-embedded tumor tissue block

Other Acceptable Specimens

5 unstained (minimum 5-micron thick sections) tumor slides (non-baked), and 1 H&E stained slide

Instructions

At least 40% tumor is required for this assay. In general, a 6 mm x 3 mm area of tissue cut at 5-micron thickness is the minimum amount of tissue needed. Tissue should be collected over multiple slides.

(Acceptable): Submit one slide stained with hematoxylin and eosin and five unstained, nonbaked 5-micron thick sections of tumor.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: Varies
Refrigerated: Not established
Frozen: Not established

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Specimens that have been decalcified (all methods) • Specimens that have not been formalin-fixed, paraffin-embedded • Bone marrow in EDTA

Methodology
Methylation PCR Analysis

Setup Schedule

Set up: Varies; Report available: 7-10 days

Report Available

10 Days

Clinical Significance

Prognostication of newly diagnosed patients with glioblastoma, IDH-wildtype.

Identifying newly diagnosed glioblastoma, IDH-wildtype patients that may respond to alkylating chemotherapy (i.e., temozolomide). Guiding therapy decision making for newly diagnosed glioblastoma, IDH-wildtype in older patients (>60-65 years).

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.