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HEPATITIS C ANTIBODY SCREEN WITH REFLEX TO HCV PCR
MessageUseful for: Detecting and diagnosing chronic hepatitis C virus (HCV) infection
If the HCV antibody screen is reactive, then HCV RNA by PCR, Quantitative (test code HCVQR) will be performed at an additional charge.
If the HCV antibody screen is reactive, then HCV RNA by PCR, Quantitative (test code HCVQR) will be performed at an additional charge.
Test Code
LAB304734
Preferred Specimen
One 7 mL lavender top tube
Minimum Volume
3.5 mL blood
Required for Testing: 1.5 mL plasma
Required for Testing: 1.5 mL plasma
Instructions
Avoid freeze-thaw cycling
Note: The Fairmont campus will perform the screening test and send the confirmation test to Morgantown.
Sample preferred to be centrifuged within 2 hours, but can be stored on the red cells. Refer to specimen stabilty.
Note: The Fairmont campus will perform the screening test and send the confirmation test to Morgantown.
Sample preferred to be centrifuged within 2 hours, but can be stored on the red cells. Refer to specimen stabilty.
Transport Temperature
Frozen
Specimen Stability
For Plasma and whole blood
Ambient: 7 days Refrigerated: 14 days Frozen: 3 months
Ambient: 7 days Refrigerated: 14 days Frozen: 3 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Turbid, lipemic, grossly hemolyzed, or heat-inactivated specimens. Cadaveric specimens
Methodology
Anti-HCV Antibody: Chemiluminescent microparticle immunoassay HCV Quantitative PCR: AmpliPrep/COBAS TaqMan HCV
Setup Schedule
Sunday- Saturday
Clinical Significance
A nonreactive test result does not exclude the possibility of exposure to or infection with hepatitis C virus.
Nonreactive test results in individuals with prior exposure to HCV may be due to antibody levels being below the detection limit of this assay
or to lack of antibody reactivity to the recombinant antigens used in this assay.
Immunocompromised patients who have HCV may produce levels of antibody below the sensitivity of this assay and may not be detected as
reactive/positive.
Nonreactive test results in individuals with prior exposure to HCV may be due to antibody levels being below the detection limit of this assay
or to lack of antibody reactivity to the recombinant antigens used in this assay.
Immunocompromised patients who have HCV may produce levels of antibody below the sensitivity of this assay and may not be detected as
reactive/positive.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
Braxton County Memorial Hospital
Camden Clark Medical Center
Fairmont Medical Center
Reynolds Memorial Hospital
Summersville Regional Medical Center
St Joseph's Hospital
