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HEPARIN DEPENDENT ANTIBODY WITH SEROTONIN RELEASE REFLEX
MessageThis is the primary test used to diagnose heparin-induced thrombocytopenia (HIT) in conjunction with clinical parameters. A
serotonin release assay will be performed when resutls of this test do not correlate with clinical impression.
serotonin release assay will be performed when resutls of this test do not correlate with clinical impression.
Test Code
LAB304813
Preferred Specimen
One 6 mL RED top tube
Minimum Volume
2 1mL Aliquots of serum
Instructions
Samples must be centrifuged within two hours of collection. Serum must be removed from the red cells and put into at least two 1mL aliquots following centrifugation.
Transport Temperature
Frozen (two 1 mL aliquots)
Specimen Stability
Ambient: 2 hours
Frozen: 6 months (-70°C), freeze within 2 hours of collection.
Frozen: 6 months (-70°C), freeze within 2 hours of collection.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Tube contaminated with heparin, gross hemolysis
Methodology
Chemiluminescent immunoassay method performed on ACL AcuStar analyzers.
Setup Schedule
Monday thru Friday
Clinical Significance
Pathology will discuss positive results with provider. A reflex for SRA testing will be generated at that time.
Performing Laboratory
West Virginia University Hospital, Inc.
