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LUPUS ANTICOAGULANT SCREEN
MessageA hematocrit value >55% may lead to spurious coagulation results.
Test Code
LAB319
Preferred Specimen
Two 3 mL blue top tube
Pediatric Volume: Three 1.8 mL pediatric blue top tubes
Pediatric Volume: Three 1.8 mL pediatric blue top tubes
Minimum Volume
Required for Testing: Two 1 mL aliquots of platelet-free plasma
Instructions
Tube must be filled to the etched line. Immediately after drawing gently invert the specimen 3 to 4 times. Do not shake.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen: two 1 mL platelet-free aliquots (platelet count < 5,000)
Specimen Stability
Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)
Frozen: 6 months (-70C)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted specimen, underfilled tube, or gross hemolysis
Methodology
Lupus Anticoagulant (LAC) Screen and LAC Confirm based on Diluted Russell's Viper Venom clotting time. Silica clotting time (SCT) Screen and SCT Confirm based on direct activation of clotting.
Setup Schedule
Monday, Wednesday and Friday
Clinical Significance
This screen for lupus anticoagulants include 2 screening tests (LAC screen/confirm & SCT screen/confirm) that include confirmatory testing
for phospholipid dependence. An interpretive comment is included in the test result.
for phospholipid dependence. An interpretive comment is included in the test result.
Performing Laboratory
West Virginia University Hospital, Inc.
