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SYPHILIS SCREENING ALGORITHM WITH REFLEX, SERUM
MessagePlease see the link below under additional information for the test change update.
Test Code
LAB1230000
Preferred Specimen
Gold Top Tube
Pediatric: 2 Gold top Microtainers
Pediatric: 2 Gold top Microtainers
Minimum Volume
Required for Testing: 0.5mL
Other Acceptable Specimens
Red Top Tube
Instructions
Centrifuge within 1 hour of collection. Separate serum from cells if Red Top Tube. Aliquot 0.5mL serum
Transport Temperature
Refrigerated
Specimen Stability
Ambient: 72 hours
2-8°C for 7 days
Frozen: 30 days
2-8°C for 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Failure to fully fill tube, Gross hemolysis
Methodology
Chemiluminescent Immunoassay
Setup Schedule
Sunday-Saturday
Clinical Significance
SYPHILIS SCREENING ALGORITHM with REFLEX, Serum
This algorithm starts with a treponemal antibody screening test, and results are reported as Nonreactive or Reactive. All Reactive results are automatically reflexed to RPR (rapid plasma regain) confirmation testing with endpoint titer reported for reactive samples. Supplemental TP-PA is performed if treponemal antibody screen and RPR results are discordant. All tests utilize FDA-cleared reagents verified by WVUH laboratories.
This algorithm starts with a treponemal antibody screening test, and results are reported as Nonreactive or Reactive. All Reactive results are automatically reflexed to RPR (rapid plasma regain) confirmation testing with endpoint titer reported for reactive samples. Supplemental TP-PA is performed if treponemal antibody screen and RPR results are discordant. All tests utilize FDA-cleared reagents verified by WVUH laboratories.
| Treponemal Antibodies | RPR Result | TP-PA Result | Conclusion/Interpretation |
| Nonreactive | Not performed | Not performed | No serologic evidence of exposure to syphilis. |
| Reactive | Reactive | Not performed | Suggestive of active infection. Treponemal and non-treponemal antibodies detected. |
| Reactive | Nonreactive | Reactive | Suggestive of remote infection with treatment/resolution OR late stage infection. The presence of treponemal antibody is confirmed, but no evidence of non-treponemal antibody is detected. |
| Reactive | Nonreactive | Nonreactive | Suggestive of false positive treponemal antibody screening result. The presence of treponemal antibody is not confirmed, and there is no evidence of non-treponemal antibody detected. |
| Reactive | Nonreactive | Indeterminate | Indeterminate. The presence of treponemal antibody is equivocal, and there is no evidence of non-treponemal antibody detected. Recommend repeat testing in 4-6 weeks. |
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
Camden Clark Medical Center
St. Joseph’s Hospital
United Hospital Center
Additional Information
Test change notification
