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HEPARIN LOW MOLECULAR WEIGHT ANTI-XA, PLASMA
Test CodeLAB316
Preferred Specimen
One 3 mL blue top tube
Pediatric Volume: One 1.8 mL pediatric blue top tube.
Pediatric Volume: One 1.8 mL pediatric blue top tube.
Minimum Volume
Required: 1 mL plasma
Instructions
Tube must be filled to the etched line. Immediately after drawing gently invert the specimen 3 to 4 times. Do not shake.
Sample should be collected 4-6 hours after subcutaenous injection. Tube must be filled to etched line to perform testing.
Samples must be centrifuged at 3000rpm for 10 minutes or using a high speed centrifuge. Platelet poor plasma should be aliquoted into 1mL vials.
Courrier Samples: Freeze within one hour of collection. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Sample should be collected 4-6 hours after subcutaenous injection. Tube must be filled to etched line to perform testing.
Samples must be centrifuged at 3000rpm for 10 minutes or using a high speed centrifuge. Platelet poor plasma should be aliquoted into 1mL vials.
Courrier Samples: Freeze within one hour of collection. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen (two 1 mL plasma aliquots)
Specimen Stability
Ambient: Unstable Frozen: Freeze plasma in 2 aliquots of 1mL each
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted sample, underfilled tube, or gross hemolysis
Methodology
Chromogenic anti-Xa assay on ACL TOP
Setup Schedule
Sunday - Saturday Note: This test is now available 24/7 and does not require a call to the Hematology lab.
Clinical Significance
LMWH: Therapeutic range is 0.50-1.00 IU/mL for twice daily dosing and 1.00-2.00 U/mL for once daily dosing when the sample is obtained
4-6 hours after subcutaneous injection.
4-6 hours after subcutaneous injection.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
