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STERILE SITE CULTURE AND GRAM STAIN AEROBIC
Test CodeLAB1230008
Includes
Gram Stain, Bacterial Culture and Antibiotic Susceptibilites (when indicated). For anaerobic culture, see LAB233 ANAEROBIC CULTURE.
Preferred Specimen
DEEP STERILE SITE: Fluid, aspirated material or tissue of deep/sterile sites labeled as tospecific anatomic source should be collected for culture and submitted in a sterile container or syringe chamber. Although swabs may be used (Eswab preferred over standard aerobic culture swab), actual tissue, aspirate, or fluid is ideal.
TISSUE:Representative tissue sample in sterile leak-proff container. If unable to deliver to lab immediately, sterile saline should be added to
prevent dessication.
FLUID: Fluid in a sterile leak-proof container
ASPIRATE: Aspirate in syringe chamber or other sterile leak-proof container
SWAB: Sample non-superficial, normally-sterile site using swabs below. NOTE: Skin or mucosal surfaces are not steriles sites and
should generally be ordered as LAB503- WOUND, SUPERFICIAL/ NONSTERILE SITE, CULTURE AND GRAM STAIN. E-Swab(preferred)
TISSUE:Representative tissue sample in sterile leak-proff container. If unable to deliver to lab immediately, sterile saline should be added to
prevent dessication.
FLUID: Fluid in a sterile leak-proof container
ASPIRATE: Aspirate in syringe chamber or other sterile leak-proof container
SWAB: Sample non-superficial, normally-sterile site using swabs below. NOTE: Skin or mucosal surfaces are not steriles sites and
should generally be ordered as LAB503- WOUND, SUPERFICIAL/ NONSTERILE SITE, CULTURE AND GRAM STAIN. E-Swab(preferred)
Instructions
Deliver to Microbiology lab immediately
Transport Temperature
Ambient - 2 hrs at 20-25°C. If delayed transport to the laboratory, refrigerate (4-8°C) up to 48 hrs unless looking for N. gonorrohoea.
Specimen Stability
Unstable, deliver to lab immediately
ESWAB Samples:
Ambient: 2 hours, If delayed transport to the laboratory, refrigerate (4-8°C) up to 48 hrs unless looking for N. gonorrohoea.
N. gonorhea – Amibent for 24 hrs at 20-25°C. (See LAB304868 – Throat Culture – GC),
ESWAB Samples:
Ambient: 2 hours, If delayed transport to the laboratory, refrigerate (4-8°C) up to 48 hrs unless looking for N. gonorrohoea.
N. gonorhea – Amibent for 24 hrs at 20-25°C. (See LAB304868 – Throat Culture – GC),
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens submitted infixative (e.g. formalin, Cytolyte, etc), non-standard or dessicated swabs, unacceptable specimen source
Eswabs received after 48 hours will be rejected.
Eswabs received after 48 hours will be rejected.
Methodology
Culture
Setup Schedule
Sunday-Saturday
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
Braxton County Memorial Hospital
Camden Clark Medical Center
Jackson General Hospital
Jefferson Medical Center
Reynolds Memorial Hospital
Summersville Regional Medical Center
St Joseph's Hospital
United Hospital Center
Wetzel County Hospital