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PLATELET FUNCTION ASSAY (PFA-100)
Test CodeLAB318
Includes
Collagen/Epinephrine test andCollagen/ADP test to detect platelet dysfunction by intrinsic platelet defects or exposure to medications containing platelet inhibiting agents.
Preferred Specimen
One 3 mL blue top tube.
Pediatric tubes are NOT ACCEPTABLE because the quantity of blood is insufficient for testing.
Pediatric tubes are NOT ACCEPTABLE because the quantity of blood is insufficient for testing.
Instructions
Tube must be filled to the etched line. Immediately after drawing gently invert the specimen 3 to 4 times. Do not shake.
Whole blood sample, do not centrifuge. Sample must be received within 3 hours of collection.
Sample must be collected by venipuncture (no butterfly or syringe), using a 21G or larger needle. Sample collected by IV/Line is NOT acceptable. A separate blue top tube MUST be collected for this test (ie, the collected tube CANNOT be used for other
testing. Tube MUST be filled to the etched line.
Whole blood sample, do not centrifuge. Sample must be received within 3 hours of collection.
Sample must be collected by venipuncture (no butterfly or syringe), using a 21G or larger needle. Sample collected by IV/Line is NOT acceptable. A separate blue top tube MUST be collected for this test (ie, the collected tube CANNOT be used for other
testing. Tube MUST be filled to the etched line.
Transport Temperature
Ambient
Specimen Stability
Stability: Test must be performed within 4 hours of collection.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted specimen, underfilled tube (not to etched line), sample drawn using a butterfly needle or collected through an IV/line, clumped platelets. If sample platelet count is <75,000/uL, testing will not be performed in accordance with manufacturers guidelines. Samples with a low platelet count or hematocrit may have falsely elevated results.
Methodology
PFA-100 System
Setup Schedule
Sunday-Saturday
Clinical Significance
Prolongation of PFA closure times (CT) is seen in congenital and acquired platelet function defects (PFD) and VWD. Anemia (Hct<28%)
and thrombocytopenia (Plt<100K/uL) can elevate PFA times. Prolonged Col/Epi CT with normal Col/ADP CT suggests aspirin induced
PFD. Prolongation of both Col/Epi and Col/ADP CT may suggest a PFD not related to aspirin.
and thrombocytopenia (Plt<100K/uL) can elevate PFA times. Prolonged Col/Epi CT with normal Col/ADP CT suggests aspirin induced
PFD. Prolongation of both Col/Epi and Col/ADP CT may suggest a PFD not related to aspirin.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
Jackson General Hospital
St Joseph's Hospital
United Hospital Center
