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BORDETELLA PERTUSSIS ANTIBODIES, IGA AND IGG BY ELISA WITH REFLEX TO IMMUNOBLOT

Message
CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR and/or Bordetella pertussis Culture. However, if serology is used to assess late-stage pertussis (>4 weeks), this is the recommended test.

If Bordetella pertussis Antibody, IgA by ELISA is 1.2 IV or greater, then Bordetella pertussis IgA Immunoblot testing will be added; if Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.


Test Code
LAB1234168


Preferred Specimen
Gold top serum 1.0 mL 


Minimum Volume
0.3mL Gold Top Serum


Instructions
Specimen Preparation:Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent." 


Transport Container
ARUP Standard Transport Tube


Transport Temperature
Refrigerated


Specimen Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
New York State Clients: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1 month


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.


Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Setup Schedule
Tuesday and Friday


Report Available
1-5 days


Performing Laboratory
ARUP Laboratories



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.