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Aspergillus DNA, Qualitative, Real-Time PCR
Test Code18873
Alias/See Also
LAB1838
Includes
Aspergillus spp
A. fumigatus
A. terreus
A. fumigatus
A. terreus
Preferred Specimen
3 mL bronchoalveolar lavage or sputum collected in a plastic sterile leak-proof container or
3 mL whole blood collected in a EDTA (lavender-top) tube or
1 mL CSF collected in a plastic sterile leak-proof container or
3 mm tissue collected in a plastic sterile leak-proof container
3 mL whole blood collected in a EDTA (lavender-top) tube or
1 mL CSF collected in a plastic sterile leak-proof container or
3 mm tissue collected in a plastic sterile leak-proof container
Minimum Volume
2 mL BAL, sputum, or whole blood • 0.3 mL CSF • 3 mm tissue
Transport Temperature
Bronchoalveolar, sputum, whole blood, and CSF: Refrigerated (cold packs)
Whole blood, tissue: Frozen
Whole blood, tissue: Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Tissue received thawed
Methodology
Real-Time Polymerase Chain Reaction (PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 2-3 days
Clinical Significance
Diagnostic methods based on Real-Time PCR offer the possibility of expedient detection of Aspergillus from a variety of clinical specimens. Rapid, specific diagnosis of A. terreus is important because this organism is often refractory to amphotericin B treatment.
