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CHROMATIN (NUCLEOSOMAL) ANTIBODY
MessageTest Code
LAB1234001
Includes
Chromatin antibodies are synonymous with histone antibodies. Separate orders for these two tests are not suggested.
Preferred Specimen
Gold Top Tube
Pediatric: 2 Gold top microtainers
Pediatric: 2 Gold top microtainers
Other Acceptable Specimens
Red Top Tube
Instructions
Centrifgue within 1 hour of collection. Separate serum from cells if Red Top Tube.Aliquot 0.5 mL serum
Required for testing: 0.5 mL
Required for testing: 0.5 mL
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated 2-8°C for 7days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Failure to fully fill tube.
Methodology
Multiplex Immunoassay
Setup Schedule
Monady - Friday Dayshift
Clinical Significance
Chromatin Antibodies play a central role in the autoimmune response if systemic lupus erythematosus (SLE), and are common sub-serology
findings in other connective tissue disease workups. Chromatin antibodies recognize histone/dsDNA complexes common to cells in a
non-replicating state. Antinuclear sub-serology testing is NOT suggested unless a positive immunofluorescence result is obtained on initial ANA testing. Frequency of these antibodies in the non-diseased population is unknown and positivity does not necessarily suggest that a connective
tissue disease is present.
findings in other connective tissue disease workups. Chromatin antibodies recognize histone/dsDNA complexes common to cells in a
non-replicating state. Antinuclear sub-serology testing is NOT suggested unless a positive immunofluorescence result is obtained on initial ANA testing. Frequency of these antibodies in the non-diseased population is unknown and positivity does not necessarily suggest that a connective
tissue disease is present.
Performing Laboratory
West Virginia University Hospital, Inc.
