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VITAMIN D, 25OH (D2 D3), ALVERNO VTD25
MessageNote: This test is an alternative screening test for Vitamin D deficiency and monitoring response in patients that are not responding to supplementation.
Test Code
LAB7993
CPT Codes
82306
Preferred Specimen
1 Gold tube (SST)

Patient Preparation
None
Minimum Volume
0.5 mL (1 mL optimal)
Other Acceptable Specimens
1 Green tube (Lithium Heparin)

OR

OR
Instructions
Allow serum samples to clot at room temperature. Centrifuge for compelte separation of serum or plasma from red cells.
Transport Container
Transfer tube
Transport Temperature
Refrigerate
Specimen Stability
2-8ºC up to 7 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
- Gross hemolysis
- Lipemia
- Gross icterus
Methodology
Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)
Setup Schedule
M, W, F only
Batched; Day shift, STAT test not available
Batched; Day shift, STAT test not available
Reference Range
Vitamin D 25-OH, Total
30 - 100 ng/mL
Values for Vitamin D 25-OH D3 and D2 not established
30 - 100 ng/mL
Values for Vitamin D 25-OH D3 and D2 not established
Clinical Significance
Vitamin D non-response to therapy (25-OH Vit D2, D3) is used to evaluate the non-response to supplemental therapy. Only order after 25-Hydroxy Vitamin D levels have been tested.
Performing Laboratory
Alverno Central Lab