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NICOTINE (COTININE) METABOLITE SCREEN, SERUM
Test CodeLAB7928
CPT Codes
80302
Preferred Specimen
1 Gold tube (SST)

Patient Preparation
None
Minimum Volume
0.5 mL (1 mL optimal)
Other Acceptable Specimens
1 Red tube

Instructions
Centrifuge for complete separtion of serum from red cells. Refrigerate until tested.
Transport Container
Transfer/aliquot tube
Transport Temperature
Refrigerate
Specimen Stability
- 2-8°C up to 7 days
- Frozen up to 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Quantity Not Sufficient (QNS)
Methodology
EIA
Setup Schedule
T, Th
Batched; Day shift
Batched; Day shift
Report Available
Same day tested
Reference Range
<50 ng/mL = Negative
>50 ng/mL = Positive
Indeterminate - LC/MS confirmation is recommended
>50 ng/mL = Positive
Indeterminate - LC/MS confirmation is recommended
Clinical Significance
Nicotine is redily metabolized into cotinine. This assay is intended as an aid in the detection of cotinine after use or exposure to tobacco products.
Performing Laboratory
Alverno Central Lab