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HEPARIN INDUCED THROMBOCYTOPENIA (HIT) ANTIBODY w/REFLEX to SRA
Test CodeLAB8336
Alias/See Also
PF4
CPT Codes
86022
Includes
If positive, this test will automatically reflex to a Serotonin Release Assay (SRA). SRA will be sent to and performed by ARUP #2005631
Preferred Specimen
1 Blue tube (Sodium Citrate)
Patient Preparation
None
Minimum Volume
1 mL (2 mL optimal)
Instructions
Centrifuge for complete separation of plasma from red cells. Plasma should be removed WITHIN 1 hour from coolection and placed in a transfer tube. If testing cannont be performed within 4 hours of collection, plasama should be frozen.
Transport Container
Transfer tube
Transport Temperature
Room temperature or Frozen
Specimen Stability
- 15-30°C up to 4 hours
- -20°C up to 6 months - frozen plasma is the preferred sample type
- Note: Up to two (2) freeze/thaw cycles will be accepted
Methodology
Qualitative Immunoturbidimetric
Setup Schedule
M, T, W, Th, F, Sa, Sun
Batched once per shift
Batched once per shift
Report Available
Same day tested
Reference Range
Negative
Although a positive result may indicate the presence of heparin-associated antibodies, it does not confirm the diagnosis of HIT as some patients may have naturally occurring antibodies to PF4.
Although a positive result may indicate the presence of heparin-associated antibodies, it does not confirm the diagnosis of HIT as some patients may have naturally occurring antibodies to PF4.
Clinical Significance
HIT antibody testing is used to aid in the diagnosis of heparin induced thrombocytopenia. Testing should be used in conjunction with the patient’s 4Ts score for pretest probability.
Heparin Induced Thrombocytopenia (HIT) occurs when Unfractionated and Low Molecular Weight Heparin treatments cause an autoimmune reaction which will trigger antibodies to activate platelets and initiate the formation of blood clots, resulting in venous and/or arterial thrombosis. PF4-H antibody is the most critical antibody in patients with HIT. Its high affinity for heparin forms an immunocomplex with the drug which then leads to platelet activation. Determining the risk of HIT can prevent unnecessary changes in anticoagulant therapy in most HIT-suspected cases. This can be done by performing a clinical assessment of HIT using the 4Ts score.** If the 4Ts score yields a score between 5 and 8, follow up with HIT-Ab testing. If the HIT-Ab testing is positive, the test will be confirmed with a Serotonin Release Assay (SRA) to confirm the diagnosis of HIT.
Heparin Induced Thrombocytopenia (HIT) occurs when Unfractionated and Low Molecular Weight Heparin treatments cause an autoimmune reaction which will trigger antibodies to activate platelets and initiate the formation of blood clots, resulting in venous and/or arterial thrombosis. PF4-H antibody is the most critical antibody in patients with HIT. Its high affinity for heparin forms an immunocomplex with the drug which then leads to platelet activation. Determining the risk of HIT can prevent unnecessary changes in anticoagulant therapy in most HIT-suspected cases. This can be done by performing a clinical assessment of HIT using the 4Ts score.** If the 4Ts score yields a score between 5 and 8, follow up with HIT-Ab testing. If the HIT-Ab testing is positive, the test will be confirmed with a Serotonin Release Assay (SRA) to confirm the diagnosis of HIT.
Performing Laboratory
Alverno Central Lab
ARUP if reflexed
