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COLD AGGLUTININS
Test CodeLAB8415 (MISCELLANEOUS)
CPT Codes
86157
Preferred Specimen
1 Red tube

Patient Preparation
Fasting at least 8 hours preferred.
Minimum Volume
0.6 mL (1 mL optimal)
Other Acceptable Specimens
None
Instructions
Warm at 37 degrees for 15 minutes, gently inverting several times. Centrifuge and remove serum from red cells. Store at room temperature until testing.
Transport Container
Red tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature up to 7 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Severe hemolysis interferes with the test and specimen will be rejected
Methodology
Hemagglutination
Setup Schedule
M, T, W, Th, F, Sa Sun
Clinical Significance
Determines the presence of cold reacting antibodies which may indicate atypical pneumonia infection with M. Pneumoniae. Test must incubate overnight. A fasting specimen is preferred to minimize the potential for turbidity due to triglycerides.
Performing Laboratory
Alverno Central Lab