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ANTINUCLEAR ANTIBODY by IFA w/REFLEX to MULTIPLEX FLOW IMMUNOASSAY
Test CodeLAB8449
Alias/See Also
ANABZ
CPT Codes
86039, If reflexed (86235 x10 & 86225)
Includes
Titer and Pattern. If test is reflexed, the quantitative detection of dsDNA, semi-quantitative detection of Chromatin, Ribosomal P, SS-A, SS-B, Sm, SmRNP, RNP, Scl-70, Jo-1, and Centromere B.
Preferred Specimen
Gold Tube (SST)
Patient Preparation
None
Minimum Volume
2 mL
Other Acceptable Specimens
None
Instructions
Centrifuge for complete separation of serum from red cells. Refrigerate until tested.
Transport Container
Gold Tube
Transport Temperature
Refrigerate
Specimen Stability
- 2-8°C up to 2 days; otherwise freeze
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
- Hemolyzed
- Lipemia
Methodology
IFA, if reflexed Multiplex Flow Immunoassay
Setup Schedule
M, W, F (day shift only)
Note: if reflex is warranted, it will be performed on the next testing day
Note: if reflex is warranted, it will be performed on the next testing day
Report Available
Results may take up to 72 hours before reported
Reference Range
<1:40
Interpretation differs based on pattern observed
Interpretation differs based on pattern observed
Clinical Significance
This test panel starts with IFA where cytoplasmic and nuclear immunofluorescence patterns on Hep-2 cells are used to detect the presence of antibodies associated with systemic autoimmune diseases which may be helpful in the diagnosis of Systemic Lupus Erythematosis, Scleroderma, Mixed Connective Tissue disease, Undifferentiated Connective Tissue disease, Sjogren's syndrome, Dermatomyositis, Polymyositis, primary biliary cirrhosis and auto immune hepatitis.
If the titer of any pattern identified is ≥1:80, Bioplex testing will be performed and all antibodies will be reported. The Bioplex method utilizes a multiplex bead assay to quantitatively detect Chromatin, Ribosomal P, SS-A 60, SS-A 52, SS-B, Sm, SmRNP, RNP A, RNP 68, Scl-70, Jo-1 and Centromere B. Proprietary software is used to report an association between the antibodies, patterns, and diseases. Positive IFA titers of 1:40 can be deteted in up to 32% of healthy individuals, stressing the importance of clinical correlation with patient signs and symptoms.
If the titer of any pattern identified is ≥1:80, Bioplex testing will be performed and all antibodies will be reported. The Bioplex method utilizes a multiplex bead assay to quantitatively detect Chromatin, Ribosomal P, SS-A 60, SS-A 52, SS-B, Sm, SmRNP, RNP A, RNP 68, Scl-70, Jo-1 and Centromere B. Proprietary software is used to report an association between the antibodies, patterns, and diseases. Positive IFA titers of 1:40 can be deteted in up to 32% of healthy individuals, stressing the importance of clinical correlation with patient signs and symptoms.
Performing Laboratory
Alverno Central Lab
