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ANTINUCLEAR ANTIBODY QUANTITATIVE TITER by IFA
Test CodeANAPR (OE), ANAIF (LAB)
Alias/See Also
ZANQT
CPT Codes
86039
Includes
Note: this test does not include a screen; ANA by IFA only
Preferred Specimen
Gold Tube (SST)
Patient Preparation
None
Minimum Volume
1 mL
Other Acceptable Specimens
None
Instructions
Centrifuge for complete separation of serum from red cells. Refrigerate until tested.
Transport Container
Gold Tube
Transport Temperature
Refrigerate
Specimen Stability
- 15-30°C up to 8 hours
- 2-8°C up to 2 days; otherwise freeze
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
- Hemolyzed
- Lipemia
Methodology
IFA
Setup Schedule
M, W, F (day shift only)
Batched; once per day run
Batched; once per day run
Reference Range
<1:40
Clinical Significance
This test utilizes cytoplasmic and nuclear immunofluorescence patterns on HEp-2 cells to detect the presence of antibodies associated with systemic autoimmune diseases which may be helpful in the diagnosis of: systemic lupus erythematosus, scleroderma, mixed connective tissue disease, undifferentiated connective tissue disease, Sjogren’s syndrome, dermatomyositis, polymyositis, primary biliary cirrhosis and auto immune hepatitis.
The test is intended for diagnostic use and is of limited clinical utility in monitoring disease course. Overlap may occur between patterns, antibodies and diseases. If a negative result is obtained in the presence of clinical signs and symptoms suggestive of systemic autoimmune disease, repeat testing using an alternative method is suggested (Multiplex bead testing/Bioplex). Positive IFA titers of 1:40 can be detected in up to 32% of healthy individuals, stressing the importance of clinical correlation. with patient signs and symptoms.
The test is intended for diagnostic use and is of limited clinical utility in monitoring disease course. Overlap may occur between patterns, antibodies and diseases. If a negative result is obtained in the presence of clinical signs and symptoms suggestive of systemic autoimmune disease, repeat testing using an alternative method is suggested (Multiplex bead testing/Bioplex). Positive IFA titers of 1:40 can be detected in up to 32% of healthy individuals, stressing the importance of clinical correlation. with patient signs and symptoms.
Performing Laboratory
Alverno Central Lab
