FACTOR VIII:C ACTIVITY

Test Code

FACT8

Alias/See Also

Antihemophilic Factor (AHF)

CPT Codes
85240

Preferred Specimen

Blue Tube (Sodium Citrate)

If a butterfly needle is use for collection, a NON-ADDITIVE discard tube must be drawn first

Minimum Volume

2 mL (Tube MUST be filled to nominal line for proper whole blood to anticoagulant ratio – see volume guides below)

Instructions

Upon collection, mix by gentle inversion 6 times. Must be processed within 4 hours. Separate and freeze plasma if unable to process within 4 hours.

Lab note: Refer to document below (Obtaining a Platelet-Poor Specimen)

Transport Container

Blue Tube

Transport Temperature

Room Temperature

Specimen Stability

15-30°C up to 4 hours
Frozen at -20° C: 3 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Serum
EDTA plasma
Clotted
Hemolyzed

Methodology
Electromagnetic Mechanical Clot Detection

Setup Schedule

DAILY, as received

Report Available

STAT: <60 minutes
Routine: <4 hours

Reference Range

52%-132%

Clinical Significance

Order to diagnose hemophilia A, acquired factor VIII deficiency, or as part of a diagnostic workup for von Willebrand disease (VWD). Also useful for monitoring treatment in patients with factor VIII deficiency or VWD. Not recommended when screening for thrombophilia.

Performing Laboratory
Mount Sinai Hospital

Additional Information
BD Vacutainer Blue Top Volume Guide
Greiner Vacutainer Blue Top Volume Guide
Obtaining A Platelet-Poor Specimen

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.