KLEIHAUER-BETKE TEST

Test Code
KLEI (OE), HPFH (LAB)


CPT Codes
85460

Preferred Specimen
Lavender Tube (EDTA)

 


Patient Preparation
None

Minimum Volume
5 mL


Other Acceptable Specimens
Pink Tube (EDTA)


Instructions
GENTLY MIX BY INVERSION MINIMUM VOLUME 1 ML


Transport Container
LAVENDER TOP TUBE


Transport Temperature
Room Temperature


Specimen Stability
  • 15-30°C up to 24 hours
  • 2-8°C up to 3 days


Methodology
Acid-elution

Setup Schedule
DAILY, as received


Report Available
24 hours TAT


Reference Range
Male/Female Ages >=12 Years: Normal Range: 0-1 %


 
RhIG Dosage for Fetomaternal Hemorrhage
%Fetal Cells Fetal Bleed mL Vials to Inject ug (mcg) IU
0 - 0.29     0 - 0.14 1 300 1500
0.3 - 0.89   15 - 44.5 2 600 3000
0.9 - 1.49   45 - 74.5 3 900 4500
1.5 - 2.09   75 - 104.5 4 1200 6000
2.1 - 2.69 105 - 134.5 5 1500 7500
 

Rh immune globulin should be given within 72 hours of delivery or the event instigating the fetal maternal bleed.  The above dosing recommendations are based on AABB standards.  Please consult with Pharmacy for further recommendations regarding potential adverse drug affects, method, timing of administration, and possible alternative formulations for higher doses.
 


Clinical Significance
This test detects and quantifies the percent of fetal cells in the mother's circulation. It is used to determine dosage of Rh immune globin (Rho-GAM) required to prevent Rh sensitization of an Rh negative mother.  It is also used as an aid in identifying fetal-maternal hemorrhage to evaluate possible trauma to the fetus.


Performing Laboratory
Mount Sinai Hospital



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.