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ABO & RH Typing
Test CodeLAB549
Alias/See Also
ABO Grouping and Rh Typing , Blood Grouping , Blood Type , Rh Type
CPT Codes
86900; 86901
Includes
Patient blood type
Preferred Specimen
Pink Tube (EDTA)


Minimum Volume
1mL (6mL optimal)
Other Acceptable Specimens
Lavender Tube (EDTA)


Transport Container
One Pink Tube
Transport Temperature
Room Temperature
Specimen Stability
Store at 1-6 °C if not tested immediately. Sample can be tested for up to three days after collection.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimen drawn in wrong tube; grossly hemolyzed specimen; improperly labeled; quantity not sufficient for analysis
Methodology
Hemagglutination
Setup Schedule
Daily, 24 hours
Report Available
Typically 30 minutes
Limitations
Interference may be due to abnormal plasma proteins, cold autoagglutinins, recent transfusion, leukemia or malignancy, positive direct Coombs' test, and some bacteria. Transfusion of large amounts of nongroup-specific plasma or packed red cells may interfere with interpretation of blood typing results. Certain antigens in the ABO system may not be fully developed in the neonate. Maternal-fetal bleed may interfere with interpretation.
Clinical Significance
Determine blood type for prenatal work-up and for cord bloods; work-up candidacy for Rho(D) immune globulin; as part of type and screen for any patient who bleeds and needs a blood transfusion.
Performing Laboratory
Mount Sinai Hospital
Holy Cross Hospital