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VITAMIN D 25 OH (HYDROXY) TOTAL
Test CodeVITD25 (OE), VITDT (LAB)
CPT Codes
82306
Preferred Specimen
1 Gold tube (SST)

Patient Preparation
None
Minimum Volume
0.5 mL (1 mL optimal)
Instructions
Centrifuge for complete separation of serum from red cells. Refrigerate until tested.
Transport Container
Transfer tube
Transport Temperature
Refrigerated
Specimen Stability
2-8°C up to 7 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
- Hemolyzed
- Lipemic
Methodology
Chemiluminescent Immunoassay (CIA)
Setup Schedule
M, T, W, Th, F
As received; STAT testing not available
As received; STAT testing not available
Report Available
Same day received
Reference Range
Deficiency | <20 ng/mL |
Insufficiency | 20-29.9 ng/mL |
Sufficiency | 30-100 ng/mL |
Toxicity | >100 ng/mL |
Clinical Significance
The total 25-OH Vitamin D level includes the D2 and D3 forms as part of the total result. It is the predominant circulating form of Vitamin D and is considered to be the most reliable index of a person's Vitamin D nutritional status. It reflects Vitamin D produced cutaneously and that obtained from food and supplements.
This test is appropriate for initial quantitation of Vitamin D in patients who are at high risk for deficiency, including obesity, osteoporosis, and chronic conditions affecting Vitamin D metabolism and for following patients on supplemental therapy.
This test is appropriate for initial quantitation of Vitamin D in patients who are at high risk for deficiency, including obesity, osteoporosis, and chronic conditions affecting Vitamin D metabolism and for following patients on supplemental therapy.
Performing Laboratory
Alverno Central Lab