VITAMIN D 25 OH (HYDROXY) TOTAL

Test Code
VITD25 (OE), VITDT (LAB)


CPT Codes
82306

Preferred Specimen
1 Gold tube (SST)


Patient Preparation
None

Minimum Volume
0.5 mL (1 mL optimal)


Instructions
Centrifuge for complete separation of serum from red cells. Refrigerate until tested. 


Transport Container
Transfer tube


Transport Temperature
Refrigerated


Specimen Stability
2-8°C up to 7 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
  • Hemolyzed
  • Lipemic


Methodology
Chemiluminescent Immunoassay (CIA)

Setup Schedule
M, T, W, Th, F

As received; STAT testing not available


Report Available
Same day received


Reference Range
Deficiency <20 ng/mL
Insufficiency 20-29.9 ng/mL
Sufficiency 30-100 ng/mL
Toxicity >100 ng/mL


Clinical Significance
The total 25-OH Vitamin D level includes the D2 and D3 forms as part of the total result.  It is the predominant circulating form of Vitamin D and is considered to be the most reliable index of a person's Vitamin D nutritional status.  It reflects Vitamin D produced cutaneously and that obtained from food and supplements. 

This test is appropriate for initial quantitation of Vitamin D in patients who are at high risk for deficiency, including obesity, osteoporosis, and chronic conditions affecting Vitamin D metabolism and for following patients on supplemental therapy.


Performing Laboratory
Alverno Central Lab



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.