AMMONIA, PLASMA

Test Code
AMON


CPT Codes
82140

Preferred Specimen
Green Tube (Lithium Heparin)



Patient Preparation
None

Minimum Volume
1 mL (2mL optimal)


Other Acceptable Specimens
Lavender Tube (EDTA)


Instructions
Fill collection tube completely and immediately place on ice. Centrifuge the sample as soon as possible and seperate plasma from cells. Aliquot into a transfer tube then freeze.

Blood should be collected into heparin or EDTA tubes, separated immediately, and the plasma kept on ice until analysis. Ammonia is stable in plasma for a maximum of 3 hours under these conditions. If sample separation from cells cannot be achieved, the sample should be kept on ice until submission to the laboratory, however ammonia will be less accurate


Transport Container
GREEN Tube


Transport Temperature
Frozen


Specimen Stability
2-4°C stable 3 hours; Frozen stable 24 hours


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
The following conditions will result in a rejected sample:

  • Hemolyzed

  • Frozen whole blood

  • Plasma submitted room temperature

  • Plasma submitted >3 hours refrigerated

  • Plasma seperator (Pearl top) tubes will be rejected




Methodology
Direct Enzymatic

Setup Schedule
DAILY, as received


Report Available
STAT: <60 minutes
Routine: <4 hours


Reference Range
Male/Female Ages >=0 Days - <1 Day: Normal Range: 90-150 umol/L Male/Female Ages >=1 Day - <14 Days: Normal Range: 79-129 umol/L Male/Female Ages >=14 Days - <1 Month: Normal Range: 29-70 umol/L Male/Female Ages >=1 Month - <16 Years: Normal Range: 15-40 umol/L Male/Female Ages >=16 Years: Normal Range: 16-53 umol/L
 Reference ranges may vary based on performing lab. Use lab reports when interpreting patient results.


Clinical Significance
Ammonia is a waste product formed primarily by bacteria in the intestines during the digestion of protein. Excess ammonia can accumulate in the blood if it is not processed and cleared from the body.



Elevated ammonia levels can be caused by severe liver disease, kidney failure, Reye syndrome or a rare genetic disorder of the urea cycle.


Performing Laboratory
Mount Sinai Hosptial 
Holy Cross Hospital



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.