ACTH STIMULATION

Test Code

ACTH STIM (OE), SACTHT (LAB)

CPT Codes
80400, 82533

Includes

Baseline cortisol (pre-injection)
30 minute cortisol (post-injection)
60 minute cortisol (post-injection)

Preferred Specimen

Gold Tube (SST)

Patient Preparation
The test consists of (3) phlebotomy draws:

Draw at baseline before Cosyntropin injection
Draw 30 minutes after Cosyntropin injection
Draw 60 minutes after Cosyntropin injection

Minimum Volume

1 mL

Other Acceptable Specimens

Green Tube (Lithium Heparin)

Instructions

Allow specimen to clot completely at room temperature. Centrifuge and separate serum or plasma from red cells ASAP or within 2 hours of collection. Specimen must be labeled with time drawn.

Transport Container

GOLD Tube

Transport Temperature

Room Temperature

Specimen Stability

2-8°C up to 2 days; 15-30°C up to 8 hours; Frozen samples are stable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolyzed samples will be rejected

Methodology
Chemilluminescence

Setup Schedule

M, T, W, Th, F, Sa, Sun

As received; STAT testing is NOT available

Reference Range

A normal response is defined as a cortisol level of >18-20 ug/dL at any time OR an increment of >7.3 ug/dL from the baseline cortisol level.

Clinical Significance

Adrenal function test; Aid in diagnosis of Addison and Cushing diseases and mulfunction of hypothalamic-pituitary-adrenal cortex axis.

Performing Laboratory
Alverno Central Lab

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.