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Adenovirus Antigen, DFA
Test Code8355
CPT Codes
87260
Preferred Specimen
3 mL of nasopharyngeal aspirate/wash, bronchial lavage/wash, throat swab, conjunctival swab
or nasal/nasopharyngeal swab in VCM medium (green-cap) tube or equivalent
or nasal/nasopharyngeal swab in VCM medium (green-cap) tube or equivalent
Minimum Volume
1 mL
Other Acceptable Specimens
Raw (unpreserved) specimen in sterile, screw-cap container
Instructions
To maintain optimum viability, place swab or fluid into VCM (equal volumes of fluid and VCM) or equivalent and transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8 degrees C or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens in VCM or equivalent should be frozen at -70 degrees C or colder and transported on dry ice. Storage or transport at -20 degrees C is not acceptable. Raw (unpreserved) samples should only be refrigerated and not frozen.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
V-C-M medium (green-cap) tube or equivalent
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Raw (unpreserved) specimen
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Raw (unpreserved) specimen
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Throats on <2 year old patients • Specimens in non-viral transport medium such as nucleic acid or bacterial transport media • Non-respiratory or non-conjunctival specimens • Dry swabs • Specimen in formalin or other fixatives • Received room temperature or frozen at -20° C
Methodology
Direct Immunofluorescence Assay (DFA)
Setup Schedule
A.M.
Report Available
Reports in 1 to 2 days.
Clinical Significance
Adenovirus causes respiratory tract infections, conjunctivitis, and diarrhea. Infections are most common in individuals who are immunocompromised and in young children. Adenovirus antigen detection is useful to confirm the diagnosis of adenovirus infection in patients with respiratory illness.