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Culture, Viral, Non-Respiratory, Conventional, Body Fluids or Tissue
Test Code689
CPT Codes
87252
Includes
Adenovirus, Cytomegalovirus, Herpes Simplex Virus Type 1, Herpes Simplex Virus Type 2, Enterovirus, and Varicella Zoster Virus
For respiratory samples please consider using the following offerings:
14867 - Viral Respiratory Rapid Culture with Reflex
14860 - Respiratory Virus Screen, DFA with Reflex to ID
91246 - Respiratory Virus Panel PCR Panel I
91233 - Respiratory Virus Panel PCR Panel IV
For respiratory samples please consider using the following offerings:
14867 - Viral Respiratory Rapid Culture with Reflex
14860 - Respiratory Virus Screen, DFA with Reflex to ID
91246 - Respiratory Virus Panel PCR Panel I
91233 - Respiratory Virus Panel PCR Panel IV
Preferred Specimen
2 mL sterile body fluids or 1 gram tissue (with saline) in VCM medium (green-cap) tube or equivalent
Minimum Volume
1 mL
Other Acceptable Specimens
Sterile body fluid or tissue (with saline) in sterile screw-cap container, plastic vial or tube
Instructions
Sterile body fluids: For all body fluids add an equal volume of specimen to transport medium (VCM or equivalent).
CSF in sterile container (without transport media): PCR is the preferable method for detection of HSV and Enterovirus.
Tissue and biopsy material: Sterile screw-capped container with small amount of saline, no fixative or preservative. Requested volume is as much as possible.
CSF in sterile container (without transport media): PCR is the preferable method for detection of HSV and Enterovirus.
Tissue and biopsy material: Sterile screw-capped container with small amount of saline, no fixative or preservative. Requested volume is as much as possible.
Transport Temperature
VCM or equivalent: Frozen -70° C (dry ice)
Raw (unpreserved) specimens: Refrigerated (cold packs)
Raw (unpreserved) specimens: Refrigerated (cold packs)
Specimen Stability
VCM medium (green-cap) tube or equivalent
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Raw (unpreserved) specimens
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Raw (unpreserved) specimens
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Wooden shaft and calcium alginate swabs • Dry swabs • Transwabs or swabs in transport medium other than specified • Tissue in formalin or other fixatives • Whole blood • Bone marrow in EDTA (refer for PCR testing) • Semen • Sputum • Respiratory specimens • Urine • Conjunctival, oral or genital lesion • Skin lesion
Methodology
Immunofluorescence Assay (IFA) • Tissue Culture
Setup Schedule
Set up: Mon-Sat; Report Available: 2-24 days
Clinical Significance
Viral isolation in tissue culture remains the most sensitive method or "gold standard" for the detection of many common viruses. Successful isolation depends on the selection of the appropriate cell lines based on provisional diagnosis or symptoms and source of infection.
