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FISH, FUS-BA 16p11.2
Test Code34012
CPT Codes
88271 (x2), 88275
Includes
If results are not possible from the submitted specimen, the test order will be cancelled and replaced by non-orderable test Cytogenetics Communication.
Preferred Specimen
Formalin-fixed paraffin-embedded tissue block
Minimum Volume
3 unstained 5 micron slides on charged (+) slides
Other Acceptable Specimens
5 slides unstained 5 micron slides on charged (+) slides
Instructions
Do not reject specimens, send to laboratory for screening.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Transport Temperature
Room temperature
Specimen Stability
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Sets up 7 days a week.
Clinical Significance
The dual color break apart FUS probe (16p11) is useful for definitive identification of rearrangements in cells from cultures or FFPE. FUS is involved in translocations with several partners and is implicated in leukemias and solid tumors. The myxoid-round cell liposarcoma is characterized by the presence of the reciprocal chromosomal translocation t(12;16)(q13;p11). This translocation creates the DDIT3-FUS chimeric gene and is associated with poor prognosis. FUS has other translocation partners that will be detected by this break apart probe, and it can be useful for investigating rearrangements involving the 16p11 region. This test can be used to assist in diagnosis and response to therapy. Adipose tumors pose difficulties for diagnosis based on histology and clinical features. The differential morphological diagnosis of soft tissue tumors is often complex, including benign versus malignant and sarcoma versus other neoplasms. Liposarcomas are the most frequent soft tissue saracomas that occur in adults. FISH probes can be helpful in differentiating between benign tumors and malignant liposarcoma and in defining the subtype of liposarcoma.
