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FISH, RET
Test Code33485
CPT Codes
88271 (x2), 88274
Includes
If results are not possible from the submitted specimen, the test order will be cancelled and replaced by non-orderable test Cytogenetics Communication.
Preferred Specimen
Formalin-fixed paraffin-embedded tissue block
Minimum Volume
3 slides unstained 5 micron slides on charged (+) slides
Other Acceptable Specimens
5 slides unstained 5 micron slides on charged (+) slides
Instructions
Do not reject specimens, send to laboratory for screening.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Transport Temperature
Room temperature
Specimen Stability
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
A.M. Sets up 7 days a week.
Clinical Significance
The RET red/green FISH probe mixture allows for definitive identification of the rearrangement in FFPE tumor specimens. RET rearrangements have been identified in 1-2% of lung adenocarcinomas (non-small cell lung cancer), especially in a younger population (<30) which are negative for EGFR, KRAS, ROS1, and ALK abnormalities. Lung cancer studies have shown these patients responding to treatments with tyrosine kinase inhibitors (TKI) such as cabozantinib. Also, RET rearrangements have been found in radiation induced papillary and medullary thyroid carcinomas.
