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Heparin, Anti-Xa
Test Code30292
CPT Codes
85520
Preferred Specimen
2 mL frozen plasma collected in 3.2% sodium citrate (light blue-top) tube
Minimum Volume
1 mL
Instructions
Platelet Poor Plasma: Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and ship on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received room temperature • Received refrigerated
Methodology
Chromogenic Assay
Setup Schedule
Night Sets up 6 days a week.
Clinical Significance
This assay is useful in measuring heparin concentration in patients treated with low molecular weight heparins (i.e. Enoxaparin/Lovenox) or unfractionated heparin. It is not intended for measurement of Rivaroxaban (Xarelto) or Fondaparinux (Arixtra).
Refer to test code 90981 for Rivaroxaban measurement and test code 16103 for Fondaparinux measurement.
Refer to test code 90981 for Rivaroxaban measurement and test code 16103 for Fondaparinux measurement.
