Thrombotic Marker Panel

Test Code

11345

CPT Codes
83520 (x2), 85362, 85379

Includes

D-Dimer, Quantitative
Fibrin Monomer
Prothrombin Fragment 1.2
Thrombin- Antithrombin (TAT) Complex

Preferred Specimen

2 mL frozen platelet-poor plasma collected in each of three separate 3.2% sodium citrate (light blue-top) tubes

Minimum Volume

1 mL each specimen

Instructions

Please submit a separate, frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Freeze immediately and transport on dry ice.

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 21 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Received refrigerated • Received room temperature

Methodology
Enzyme Immunoassay (EIA) • Enzyme Linked Immunosorbent Assay (ELISA) • Hemagglutination (HA) • Immunoturbidimetric

Setup Schedule

See individual assays

Clinical Significance

When intravascular thrombosis is suspected in the absence of overt clinical symptoms, elevation in D-dimer, fibrin monomer, prothrombin fragment 1.2, or thrombin-antithrombin complex may be the only indicator of intravascular clot formation and generation of activated factor X. Marked elevation in only 1 or 2 of these markers should lead to further investigation of the site of the thrombosis (eg, early dissecting aortic aneurysm, anomalous arterial/venous fistula).

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.