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Antimicrobial Susceptibility, Anaerobic Bacteria, 3 Drug, MIC
Test Code90279
CPT Codes
87181 (x3)
Preferred Specimen
Pure culture of anaerobic isolate
Instructions
Transport in an anaerobic environment; slant, plate, or swab. Collect swab specimen using an Amies gel transport swab, Amies liquid elution swab (ESwab), or equivalent. Isolate identification must be supplied with the request.
Refrigerated preferred and may help improve isolate viability.
Refrigerated preferred and may help improve isolate viability.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Determined by viability
Refrigerated: Determined by viability
Frozen: Unacceptable
Refrigerated: Determined by viability
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Isolate in thioglycollate broth • Aerobic slant, plate or swab • Frozen isolates (unless in glycerol broth - acceptable)
Methodology
Gradient Diffusion
Setup Schedule
Set up: Daily; Report available: 4-5 days
Clinical Significance
Resistance of anaerobic organisms to antibiotics has been increasing in recent years. Significant increases in resistance are now seen with non-bacteroides fragilis group gram-negatives and other genera such as clostridium, fusobacterium, prevotella, and peptostreptococcus. Since negative therapeutic outcomes are directly associated with antimicrobial resistance, accurate, timely susceptibility testing of anaerobes has become more important.
