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Surface Light Chains (11015X)
Test Code11015N
CPT Codes
88184, 88185
Preferred Specimen
5 mL whole blood, or 2 mL bone marrow collected in a sodium heparin (green-top) tube, or fresh smear
Minimum Volume
1 mL
Other Acceptable Specimens
EDTA (lavender-top) tube, or ACD solution B (yellow-top) tube • Fresh (unfixed) tissue • CSF • Body fluid • Pericardial fluid • Peritoneal fluid • Pleural fluid
Instructions
Submit green-top/yellow-top whole blood or 2 mL heparinized bone marrow.
A freshly prepared smear (stained or unstained) is also recommended.
A clinical summary or differential diagnosis is required along with specimen. Place a representative portion of fresh tissue (0.5 cm square) in sterile tissue culture medium such as RPM1-1640 (medium can be obtained by calling the laboratory at extension 4341) in a sterile container. Alternatively, place the tissue on a saline-soaked gauze pad in a sealed specimen cup and ship refrigerated. Do not suspend the tissue in saline.
Please submit stained H & E slide, a copy of the pathology report and a brief clinical summary.
Ship blood/bone marrow at room temperature. Ship tissue/fluid refrigerated.
A freshly prepared smear (stained or unstained) is also recommended.
A clinical summary or differential diagnosis is required along with specimen. Place a representative portion of fresh tissue (0.5 cm square) in sterile tissue culture medium such as RPM1-1640 (medium can be obtained by calling the laboratory at extension 4341) in a sterile container. Alternatively, place the tissue on a saline-soaked gauze pad in a sealed specimen cup and ship refrigerated. Do not suspend the tissue in saline.
Please submit stained H & E slide, a copy of the pathology report and a brief clinical summary.
Ship blood/bone marrow at room temperature. Ship tissue/fluid refrigerated.
Transport Temperature
Blood, bone marrow or smear: Room temperature
Tissue or fluid: Refrigerated (cold packs)
Tissue or fluid: Refrigerated (cold packs)
Specimen Stability
Whole blood or bone marrow or fresh smear
Room temperature: 72 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Tissue or fluids
Refrigerated: 72 hours
Frozen: Unacceptable
Room temperature: 72 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Tissue or fluids
Refrigerated: 72 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen • Fixed sample
Methodology
Flow Cytometry (FC)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 2-3 days
Clinical Significance
The presence of immunoglobulin light chains (kappa or lambda) on the cell surface is characteristic of clonal proliferation most often seen in multiple myeloma and lymphoproliferative diseases.
