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Nonesterified Fatty Acids (Free Fatty Acids)
Test Code449
CPT Codes
82725
Preferred Specimen
1 mL frozen serum
Patient Preparation
Overnight fasting is preferred
Minimum Volume
0.3 mL
Other Acceptable Specimens
Serum collected in no additive (royal blue-top) tube • Plasma collected in: EDTA (lavender or royal blue-top) tube, 3.2% sodium citrate (light blue-top) tube, ACD solution B (yellow-top) tube, sodium fluoride or potassium oxalate (gray-top) tube, PTH-related protein (special collection tube), ACTH (lavender-top) tube, or PPT potassium EDTA (white-top) tube
Instructions
Serum or plasma should be removed from cells immediately after collection
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 2 hours
Frozen: 30 days
Refrigerated: 2 hours
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received thawed • Received refrigerated • Heparin plasma and patients on heparin therapy
Methodology
Spectrophotometry (SP) • Enzymatic
Setup Schedule
Night Sets up 2 days a week.
Clinical Significance
The Nonesterified Fatty Acids (Free Fatty Acids) assay is useful in evaluating lipolysis and fatty acid oxidation. Nonesterified Fatty Acids may be useful in evaluating patients with hypoglycemia and for nutritional assessment.
