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Melanoma, BRAF V600 Mutation, Cobas®
Test Code90956
CPT Codes
81210
Preferred Specimen
Formalin-fixed paraffin-embedded tissue block
Instructions
Do not freeze.
Freezing is not recommended for FFPE.
Please contact the Oncology Center at 1(800)642-4657 x 2906, prior to submission of slides for detailed instructions.
Freezing is not recommended for FFPE.
Please contact the Oncology Center at 1(800)642-4657 x 2906, prior to submission of slides for detailed instructions.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Indefinite
Refrigerated: Indefinite
Frozen: Do not freeze
Refrigerated: Indefinite
Frozen: Do not freeze
Methodology
Real-Time Polymerase Chain Reaction (Cobas®)
Setup Schedule
A.M.
Clinical Significance
This FDA-cleared real-time PCR assay on the cobas 4800 platform assesses for mutations at codon 600 of BRAF, particularly V600E, which are present in 40-60% of melanomas. Melanomas with codon 600 BRAF mutations are associated with response to the BRAF-directed kinase inhibitor vemurafenib (ZELBORAF®).
Tumor DNA is extracted from fixed tumor tissue and subjected to real-time PCR, with approximate sensitivity of 5% mutation-bearing cells in a mixed sample. Other codon 600 BRAF mutations, beside the most common V600E (c.1799T>A) may not be detected by this method.
Tumor DNA is extracted from fixed tumor tissue and subjected to real-time PCR, with approximate sensitivity of 5% mutation-bearing cells in a mixed sample. Other codon 600 BRAF mutations, beside the most common V600E (c.1799T>A) may not be detected by this method.