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Mast Cell Tryptase, IHC without Interpretation
Test Code40017X
CPT Codes
88342
Preferred Specimen
Formalin fixed paraffin embedded tissue in an IHC specimen transport kit
Other Acceptable Specimens
4-micron unstained slides (x5) • Fixed tissue
Instructions
Tumor paraffin block (formalin-fixed only) or five 4-micron unstained sections on Poly-L-Lysine or Silane coated slides.
See Specimen Collection Section, Oncology.
Ship at room temperature.
Do not place paper labels with adhesive backing on slides. Use pencil or xylene resistant pen to write on the frosted end of the slide only.
Pathology report is required.
See Specimen Collection Section, Oncology.
Ship at room temperature.
Do not place paper labels with adhesive backing on slides. Use pencil or xylene resistant pen to write on the frosted end of the slide only.
Pathology report is required.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Indefinite
Refrigerated: Indefinite
Frozen: Unacceptable
Refrigerated: Indefinite
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Tissue in formalin
Methodology
Immunohistochemistry (IHC)
Setup Schedule
A.M.
Clinical Significance
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of tryptase is reported to be found in mast cells of skin and lung and suggests this enzyme plays a major role in mast cell mediated events. In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase. Models of allergenic disease in the skin, nose and lung have each indicated elevated tryptase levels. Human mast cell tryptase has been reported to be implicated as a mediator of inflammation. Mast cell degranulation in the gut causes mucus secretion, mucosal edema, increased gut permeability and may be responsible for some of the symptoms and signs of inflammatory bowel disease.
