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Mantle Cell Lymphoma, bcl-1/JH t(11;14), Real-time PCR, Cell-based
Test Code14991
CPT Codes
81168<br />
Preferred Specimen
5 mL bone marrow collected in an EDTA (lavender-top) tube
Minimum Volume
1 mL
Other Acceptable Specimens
Whole blood collected in an EDTA (lavender-top) tube • Fresh (unfixed tissue) • Formalin-fixed, paraffin embedded tissue block • Tissue (biopsy) • CSF • Fluid
Instructions
Collect whole blood or bone marrow in an EDTA (lavender-top) tube ship refrigerated or room temperature. Stability of whole blood and bone marrow samples at room temperature is 1 week and at refrigerated temperature is 2 weeks.
Paraffin blocks are to be shipped at room temperature. Stability for paraffin blocks is indefinite. For tissue samples, fresh frozen tissue is preferred and to be shipped frozen.
Frozen tissue is stable for approximately 1 year at -70°C. Fresh (unfixed) must be in RPMI.
Tissues in fromalin fixative or RPMI are to be shipped at room temperature or refrigerated. Identify what fixative or media is used and write it on the samples container.
Paraffin blocks are to be shipped at room temperature. Stability for paraffin blocks is indefinite. For tissue samples, fresh frozen tissue is preferred and to be shipped frozen.
Frozen tissue is stable for approximately 1 year at -70°C. Fresh (unfixed) must be in RPMI.
Tissues in fromalin fixative or RPMI are to be shipped at room temperature or refrigerated. Identify what fixative or media is used and write it on the samples container.
Transport Temperature
See instructions
Specimen Stability
Whole blood or bone marrow
Room temperature: 7 days
Refrigerated: 14 days
Formalin fixed paraffin embedded tissue
Room temperature: Indefinite
Room temperature: 7 days
Refrigerated: 14 days
Formalin fixed paraffin embedded tissue
Room temperature: Indefinite
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
A.M.
Clinical Significance
Bcl-1/JH t(11;14) is highly specific for mantle cell lymphoma (MCL). The t(11;14) can be found in approximately 70% to 95% cases of MCL. Half of these translocations fall within the BCL-1 major translocation cluster (MTC). The t(11;14) Real-Time PCR assay can be used to monitor minimal residual disease in NHL. Ideally, recent patient samples should be tested side by side with the previous sample to better illustrate the quantitative changes.
