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Lactate Dehydrogenase (LD) Isoenzyme Panel
Test Code4411
CPT Codes
83615, 83625
Includes
Lactate Dehydrogenase (LD)
Lactate Dehydrogenase Isoenzymes (LD1, LD2, LD3, LD4, LD5)
Lactate Dehydrogenase Isoenzymes (LD1, LD2, LD3, LD4, LD5)
Preferred Specimen
2 mL serum
Minimum Volume
1 mL
Instructions
Centrifuge serum specimens within 1 hour of collection, transfer serum to a clean, plastic, transport tube(s), and transport at room temperature to the laboratory. Do not refrigerate or freeze the specimens.
Specimens cannot be shared, and no other tests should be ordered with Lactate Dehydrogenase Isoenzymes.
Specimens cannot be shared, and no other tests should be ordered with Lactate Dehydrogenase Isoenzymes.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received refrigerated • Received frozen
Methodology
Electrophoresis • Spectrophotometry (SP)
Setup Schedule
Night Sets up 3 days a week.
Report Available
Reports in 2 to 3 days.
Clinical Significance
Lactate dehydrogenase (LD) is present in many different organs and tissues such as liver, heart, muscles, kidney, lungs and blood. The usual designation of isoenzymes is LD-I, LD-II, LD-III, LD-IV and LD-V. LD isoenzymes tissue specificity is derived from the fact that LD subunits are synthesized in well-defined ratios and could be used in investigating unexplained causes of Total LD elevations and detection of macro-LD. Do not use LD isoenzymes to detect myocardial injury.