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Kappa/Lambda Light Chain, ISH with Interpretation
Test CodeCPT Codes
88365, 88364
Preferred Specimen
Other Acceptable Specimens
Instructions
Transport Temperature
Specimen Stability
Refrigerated: Indefinite
Frozen: Unacceptable
Methodology
In-Situ Hybridization (ISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Clinical Significance
This test is used to evaluate the genetic expression of kappa and lambda light chains in fixed lymphoid tissue or bone marrow. An atypical ratio of kappa-expressing to lambda-expressing cells can help support a diagnosis of plasma cell neoplasm or B-cell lymphoma [1,2].
Immunoglobulin comprises 4 polypeptide chains: 2 heavy chains and 2 light chains (kappa or lambda). Light chains can be found on the surface of B cells and in the cytoplasm of plasma cells. Restricted expression of kappa or lambda light chains can be an indication of B-cell lymphomas or plasma cell neoplasms, which include monoclonal gammopathy of undetermined significance, plasmacytoma, and multiple myeloma. Multiple myeloma and other plasma cell neoplasms may cause amyloidosis, which can lead to organ dysfunction [3].
This test is used to evaluate the expression of kappa and lambda light chains when fixed tissue or a limited amount of biopsy tissue is obtained. When sufficient fresh tissue is available, flow cytometry can be used to achieve high sensitivity over a broad dynamic range of protein expression. Serum kappa/lambda light chains can also be used to assist in the diagnosis of plasma cell disorders as well as in monitoring disease activity and treatment. Kappa/lambda light chain in situ hybridization cannot detect small amounts of surface immunoglobulin present on most resting B cells [1,3].
Interpretation of this test is performed by a qualified pathologist. The significance of the result should be determined in the context of pertinent clinical and family history, findings of physical examinations, and other diagnostic tests.
References
1. Guo L, et al. Mod Pathol. 2018;31(3):385-394.
2. Weiss LM, et al. Am J Pathol. 1990;137(4):979-988.
3. National Cancer Institute. Plasma cell neoplasms (including multiple myeloma) treatment (PDQ®)-health professional version. Accessed September 1, 2021. https://www.cancer.gov/types/myeloma/hp/myeloma-treatment-pdq