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Isotretinoin, Serum/Plasma
Test Code26519X
CPT Codes
80299
Preferred Specimen
1 mL serum collected in a red-top tube (no gel) - protected from light
Minimum Volume
0.3 mL
Other Acceptable Specimens
Plasma collected in light protected: EDTA (lavender-top) tube or EDTA (pink-top) tube
Instructions
Promptly centrifuge and separate serum or plasma into a light protected plastic screw capped vial using approved guidelines.
Known interferences: Isotretinoin [LC-MS/MS]:9-cis, 13-cis-retinoic acid
Known interferences: Isotretinoin [LC-MS/MS]:9-cis, 13-cis-retinoic acid
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 30 days
Refrigerated: 30 days
Frozen -20° C: 4 months
Frozen -70° C: 14 months
Refrigerated: 30 days
Frozen -20° C: 4 months
Frozen -70° C: 14 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received not protected from light • Serum Separator Tube (SST®) • PST
Methodology
High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS)
Setup Schedule
Set up: As needed; Report available: 1 day
Clinical Significance
Therapeutic Drug Monitoring
