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Iron, Total
Test Code571
CPT Codes
83540
Preferred Specimen
1 mL serum
Patient Preparation
Samples should be taken in the morning from patients in a fasting state, since iron values decrease by 30% during the course of the day and there can be significant interference from lipemia
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in a sodium heparin (green-top) or lithium heparin (green-top) tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 6 days
Refrigerated: 7 days
Frozen: 31 days
Refrigerated: 7 days
Frozen: 31 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Anticoagulants other than heparin
Methodology
Spectrophotometry (SP)
Setup Schedule
Set up: Mon-Sat; Report available: 2-3 days
Clinical Significance
Serum measurements are useful in the diagnosis of iron deficiency and hemochromatosis.