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IgG, IgA, Indirect Immunofluorescence
Test Code16690
CPT Codes
88346, 88350
Preferred Specimen
7.5 mL (x2) whole blood collected in red/black tiger-top (SST)
Minimum Volume
7.5 mL whole blood • 3 mL serum
Other Acceptable Specimens
6 mL serum collected in a red-top tube (no gel)
Instructions
Draw 7.5 mL whole blood in each of two tubes (10 mL tiger-top vacutainer or generic serum separator tube). No spin necessary. Submit a minimum of 7.5 mL of blood. If only one tube is submitted, it should be full. If two tubes are submitted, the volume of both tubes combined should add up to at least 7.5 mL of blood.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Methodology
Immunoassay (IA)
Setup Schedule
Sets up 5 days a week.
Clinical Significance
These studies are complementary diagnostic and prognostic tools for autoimmune blistering diseases, connective tissue disorders, and vasculitides. It is a semiquantitative technique whereby a double immunolabeling is performed to evaluate the presence and titer of circulating anti-epithelial cell surface, anti-basement membrane, anti-nuclear, and antineutrophil cytoplasmic antibodies.
