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Herpes Simplex Virus, Type 1 and 2 DNA, Quantitative, Real-Time PCR
Test Code19502
CPT Codes
87530 (x2)
Preferred Specimen
1 mL CSF, vitreous, pericardial, pleural, or amniotic fluid collected in a sterile, leakproof container OR
1 mL serum OR
1 mL plasma collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube
1 mL whole blood collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube
1 mL serum OR
1 mL plasma collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube
1 mL whole blood collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube
Minimum Volume
0.3 mL
Other Acceptable Specimens
1 mL bronchoalveolar lavage or bronchial wash collected in a sterile leak proof container
Transport Temperature
Whole blood: Refrigerated (cold packs)
All other specimen types: Frozen
All other specimen types: Frozen
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1-2 days
Clinical Significance
The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 100-2,000,000 copies/mL.