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Heparin-Induced Platelet Antibody
Test Code414
CPT Codes
86022
Preferred Specimen
1 mL frozen serum collected in a red-top tube (no gel)
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in: 3.2% sodium citrate (light blue-top) tube
Instructions
For serum: Centrifuge red top tube 15 minutes at approximately 1500 g as soon as possible after clotting.
For plasma: Centrifuge blue top tube 20 minutes at approximately 2500 g. Remove plasma from cells as soon as possible.
For plasma: Centrifuge blue top tube 20 minutes at approximately 2500 g. Remove plasma from cells as soon as possible.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Rrefrigerated: Unacceptable
Frozen: 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Icteric specimen • Lipemic specimen • Serum separator tube (SST®)
Methodology
Immunoassay (IA)
Setup Schedule
P.M.
Limitations
When heparin is stopped, antibody that may have been present disappears within weeks to months.
Clinical Significance
Heparin can induce thrombocytopenia, a decreased platelet count, due to antibody stimulation. False positive results may be due to immune complexes. False negative results may be due to low titer, low avidity antibodies.