Glucagon

Test Code
519

CPT Codes
82943

Preferred Specimen
2 mL plasma collected in an EDTA (lavender-top) tube

Patient Preparation
Overnight fasting is required

Minimum Volume
0.5 mL

Instructions
Collect EDTA plasma, invert several times to mix, centrifuge, and immediately transfer the plasma into a clean, plastic transport tube and freeze at -60 to -90° C. There should be no delay in freezing as the analyte is very unstable and samples must be kept frozen until testing.

Transport Temperature
Frozen -70° C

Specimen Stability
Room temperature: Unacceptable
Refrigerated: 2 hours
Frozen -20° C: 24 hours
Frozen -70° C: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Received refrigerated • Received room temperature

Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)

Setup Schedule
A.M.

Clinical Significance
The glucagon assay is useful primarily when considering a glucagon-secreting tumor of the pancreas. Glucagonomas cause an unusual but characteristic syndrome consisting of a rash, mild diabetes, weight loss and hypoamninoacidemia. Measurement of plasma glucagon confirms the diagnosis; glucagon levels are very high in the setting of glucagonoma.



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.