Follicular Lymphoma, BCL-2/JH t(14;18), Real-Time PCR, Cell-based

Test Code

15007

CPT Codes
81278<br />

Preferred Specimen

5 mL bone marrow or whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

1 mL

Other Acceptable Specimens

Fresh (unfixed) tissue • Frozen tissue • Formalin-fixed, paraffin-embedded tissue block • Tissue in neutral buffered formalin • Tissue (biopsy)

Instructions

Whole blood or bone marrow: Collect in an EDTA (lavender-top) tube. Ship refrigerated or room temperature. Do not ship frozen. Stability of whole blood and bone marrow samples at room temperature is 1 week and refrigerated is 2 weeks.

Paraffin blocks: Ship at room temperature. Stability for paraffin blocks is indefinite.

Tissue samples: Fresh frozen tissue is preferred and to be shipped frozen. Frozen tissue is stable for approximately 1 year at -70° C.

Fresh (unfixed) tissue: Must be in RPMI. Tissues in formalin fixative or RPMI are to be shipped at room temperature or refrigerated. Identify what fixative or media is used and write it on the sample container.

Transport Temperature

See Collection Instructions

Specimen Stability

⁠⁠⁠⁠⁠⁠⁠Whole blood or Bone marrow
Room temperature: 7 days
Refrigerated: 14 days
Frozen -20°C: See Collection Instructions
Frozen -70°C: 1 year

Formalin fixed paraffin embedded tissue
Room temperature: Indefinite

Fresh (unfixed) tissue, Biopsy, Fixed tissue or Tissue in neutral buffered formalin
Frozen -70°C: 1 Year

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

A.M. Sets up 1 day a week.

Clinical Significance

Follicular lymphoma is the most common type of non-Hodgkin's lymphoma in the United States. Up to 90% of follicular lymphomas carry BCL-2/JH t(14;18) (q32;Q21) chromosome translocation. The presence of the BCL-2 translocation has been associated with poor prognosis in one group of diffuse large-cell and follicular lymphoma patients. Analysis of BCL-2 translocations provides an important marker for detection and monitoring of malignancy during patient treatment and follow up.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.