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Fibrinogen Activity, Clauss (461X)
Test Code32557P
CPT Codes
85384
Preferred Specimen
1 mL frozen plasma collected in a 3.2% sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
Instructions
Platelet-poor plasma: Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and ship on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Clotted
Methodology
Photometry/Optical, Clauss
Setup Schedule
A.M. Sets up 2 days a week.
Limitations
Expected impact by therapeutic levels (potential interference depends upon drug concentration): Warfarin: no effect; Heparin (UFH or LMWH): UFH-no effect, LMWH-normal to falsely low; Dabigatran or Argatroban (Thrombin Inhibitors): no effect or falsely low; Rivaroxaban or Apixaban (Factor Xa Inhibitors): no effect.
Clinical Significance
Fibrinogen is essential for the formation of a blood clot. Deficiency can produce mild to severe bleeding disorders.
