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Factor VIII Antigen
Test Code90879
CPT Codes
85244
Preferred Specimen
1 mL frozen platelet-poor plasma collected in a 3.2% sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
Instructions
Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic screw-cap vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic screw-cap vial. Plasma must be free of platelets (<10, 000/mcl). Freeze immediately and ship on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 4 hours
Refrigerated: 48 hours
Frozen -20° C: 30 days
Frozen -70° C: 6 months
Refrigerated: 48 hours
Frozen -20° C: 30 days
Frozen -70° C: 6 months
Methodology
Immunoassay (IA)
Setup Schedule
SET UP AS NEEDED AT LEAST 1X/MO; REPORT AVAILABLE: 2-31 DAYS
Clinical Significance
An ELISA for Factor VIII antigen may be used in conjunction with the functional assays in the assessment of Factor VIII replacement therapies, assessment of carrier status, as well as to distinguish those patients that may be deemed cross-reactive material (CRM) positive.
