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Factor VIII Activity, Chromogenic
Test Code16049
CPT Codes
85240
Preferred Specimen
1 mL frozen plasma collected in 3.2% sodium citrate (light-blue top tube)
Minimum Volume
0.5 mL
Instructions
Platelet Poor Plasma: Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and ship on dry ice.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 8 hours
Refrigerated: 8 hours
Frozen: 21 days
Refrigerated: 8 hours
Frozen: 21 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature • Received refrigerated
Methodology
Chromogenic Assay
Setup Schedule
A.M. Sets up 2 days a week.
Clinical Significance
Preferred test in patients with Lupus anticoagulant, on heparin or direct thrombin inhibitors, or on Factor VIII concentrates.