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Factor IX Activity and Human Inhibitor
Test Code17845X
CPT Codes
85250, 85335
Preferred Specimen
2 mL [x2] plasma collected in 3.2% sodium citrate (light blue-top) tube
Minimum Volume
1 mL (x2)
Instructions
Platelet poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice. Do not thaw.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Frozen Only
Refrigerated: Frozen Only
Frozen: 14 days
Refrigerated: Frozen Only
Frozen: 14 days
Methodology
Clot Based Assay • Photometric Clot Detection
Setup Schedule
Set up: Tues; Report available: 3 days
Clinical Significance
Factor IX Human Inhibitor provides a quantitative determination of inhibitors. This aids in developing treatment strategies for patients with hemophilia.
