Estradiol and Estrone

Test Code

7437

CPT Codes
82670, 82679

Includes

Estradiol, Ultrasensitive, LC/MS and Estrone

Preferred Specimen

1 mL serum collected in a red-top tube (no gel)

Minimum Volume

0.45 mL

Instructions

Specify age and sex on test request form

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 24 hours
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Serum separator tube (SST) • Grossly icteric

Methodology
Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

See individual assays

Clinical Significance

This panel measures the concentrations of estrone and estradiol, the 2 most potent estrogens that are biologically active. This panel may help assess estrogen status in children and nonpregnant adults.

Estrogens are endogenous steroids that regulate growth and maintenance of sex organs and secondary sex characteristics in women. Estrogens also have other functions and effects, such as production of liver proteins, arterial vasodilation, reduction of intraocular pressure, influence on mood, and maintenance of bone density in both males and females [1]; lack of estrogens has important consequences in both men and women. Low estrogen levels in normal men receiving a gonadotropin releasing hormone agonist and an aromatase inhibitor is associated with decreased sexual function and increased body fat [2]. Measurements of estrogens may help evaluate sexual maturity, menstrual abnormalities, fertility abnormalities, fetal-placental health in pregnancy, tumors that excrete estrogens, or feminization syndromes in men [3].

Increased levels of estrogens may be observed in normal pregnancy, precocious puberty, hyperthyroidism, liver cirrhosis, and ovarian, testicular, and adrenal tumors. Decreased levels of estrogens may be observed in failing pregnancy, Turner syndrome, hypopituitarism, primary and secondary hypogonadism, menopause, polycystic ovarian disease, and anorexia nervosa [4].

Estrone levels may exhibit diurnal rhythm in postmenopausal women because of the adrenal origin of the estrone precursor (androstenedione). Estradiol levels do not have the same diurnal variation [3].

The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.

References
1. Gruber CJ, et al. N Engl J Med. 2002;346(5):340-352.
2. Finkelstein JS, et al. N Engl J Med. 2013;369(11):1011-1022.
3.Nerenz RD, et al. Reproductive endocrinology and related disorders. In: Rifai N, et al. eds. Tietz Textbook of Laboratory Medicine. 7th ed. Elservier Inc; 2022:9521-9714.
4. Pagana K, et al. Mosby's Diagnostic and Laboratory Test Reference. 15th ed. Elsevier Inc; 2020:346-400.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.